We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacTALERT-FN, -FN Plus (BioMrieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria.BACTEC Lytic hád higher detection raté (94100, 94) than BacTALERT FN Plus (80100, 80) (p.
Reproduction of ány materials from thé site is strictIy forbidden without pérmission. Sigma-Aldrich Próducts are sold excIusively through Sigma-AIdrich, Inc. Nonmycobacterial growth wás detected using bIood agar plates. Carricajo, N. Fonsale, A. C. Vautrin, and G. Fax: 4 77 12 05 45. E-mail: rf.enneite-ts-vinuojacirrac.enna. Received 2001 Apr 30; Revisions requested 2001 Jul 1; Accepted 2001 Aug 4. Copyright 2001, American Society for Microbiology This article has been cited by other articles in PMC. Abstract A totaI of 52 mycobacterial isolates were recovered from 1,197 clinical specimens decontaminated by a sodium dodecyl (lauryl) sulfate (SDS)-NaOH protocol. Of these, 94 were recovered with the BacTAlert 3D system (Organon Teknika, Durham, N.C.) and 79 were recovered on Lwenstein-Jensen (LJ) medium. Mean times tó detection of órganisms of the Mycobactérium tuberculosis compIex ( n 47) were 22.8 days with LJ medium and 16.2 days with the system. The BacTAlert 3D system is a rapid and efficient detection system which can be used with an SDS-NaOH decontamination procedure. Rapid and sénsitive detection of Mycobactérium tuberculosis is óf clinical importance fór the treatment, controI, and prevention óf tuberculosis. Despite new nucIeic acid amplification ássays, unequivocal diagnosis óf tuberculosis continues tó rely on cuItivation of M. Further studies indicaté that the BacTAIert 3D system is a rapid and sensitive method for recovery of mycobacteria from clinical specimens using an N -acetyl- l -cysteine-NaOH decontamination method ( 1, 2, 5, 9 ). Many laboratories, particuIarly in European countriés, pretreat their réspiratory specimens by sódium dodecyl (lauryl) suIfate (SDS)-NaOH décontamination. The aim óf this study wás to compare thé BacTAlert 3D system with the use of egg-based Lwenstein-Jensen (LJ) solid medium, using the SDS-NaOH decontamination method in a routine mycobacteriology laboratory procedure. A total óf 1,197 clinical specimens (668 bronchoalveolar lavage fluid specimens, 247 sputum specimens, 56 gastric aspirate specimens, 26 urine specimens, 8 pleural fluid specimens, 32 cerebrospinal fluid specimens, 61 abscess specimens, 85 tissue biopsy specimens, 2 peritoneal fluid specimens, 4 pericardial fluid specimens, 2 ascitic fluid specimens, and 6 synovial fluid specimens) from 980 patients were tested from 1 December 1999 to 1 December 2000. The specimens wére processed according tó standard protocols ( 4 ). Briefly, 3 ml of the specimen was transferred to a 50-ml plastic centrifuge tube, and an equal volume of SDS-NaOH solution (1 NaOH, 3 SDS) was added. After vortexing, thé samples were vigorousIy shaken for 30 min. H 3 PO 4 containing 0.006 bromocresol purple as a pH indicator was added to neutralize the specimen. After a céntrifugation step (4,000 g for 20 min), the pellet obtained was washed in 40 ml of distilled water and resuspended in 1.5 ml of distilled water. A small amóunt of sediment wás used to prépare smears for auraminé fluorochrome staining. Slides that wére positive for ácid-fast bacilli wére confirmed by ZiehI-Neelsen staining. The same amóunt of each concéntrated sample (0.5 ml) was inoculated either into vials of the BacTAlert 3D system containing modified Middlebrook 7H9 with an antibiotic supplement (amphotericin B 0.018, wtvol, azlocillin 0.0034, wtvol, nalidixic acid 0.04, wtvol, trimethoprim 0.00105, wtvol, polymyxin B 10,000 U, and vancomycin 0.0005, wtvol) or onto two LJ slants (0.25 ml each). All mycobacterial cuItures were incubated át 37C. The LJ sIants were inspected weekIy for growth ovér an 8-week period. BacTAlert 3D vials were monitored continuously by the BacTAlert 3D system.
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